Early Morphofunctional Changes in Rats' Liver after Left Lobe Resection using Cold Plasma Coagulator (Experimental Research)
https://doi.org/10.16931/1995-5464.2015176-83
Abstract
Aim: to assess morphofunctional changes in rats' liver after left lobe resection using cold plasma hemostasis. Material and Methods. Research was performed on 6 experimental groups with 30 laboratory rats. There were 5 intact animals in control group and 5 animals in each group on the 3rd, 5th, 7th, 14th, 30th day after liver left lobe resection and coagulation with cold plasma coagulator. The biochemical analysis, coagulogram control and morphological study
of rats’ liver were performed. Results. Analysis of biochemical markers revealed that activity of AST, α-amylase, alkaline phosphatase and levels of glucose, urea are increased in early period what indicates on liver damage. AST is reduced by the 30th day. It indicates on repair of liver parenchyma. Slight increase of α-amylase and alkaline phosphatase levels by the 30th day is associated
with continued overall reaction to surgery. There is increase of specific volume of hepatocytes with signs of degeneration, the number of cells with necrotic changes in 1 mm2, infiltrate density in 1 mm2 and specific volume of connective tissue in early period followed gradually reduce by the 30th day. These changes point out destructive process in early period and its gradual reduction by the 30th day. Specific volume of binucleated hepatocytes in early period is reduced and increased by the 30th day as a sign of reduction and further stimulation of regenerative process by the 30th day.
About the Authors
E. V. Semichev
Chair of Hospital Surgery Faculty of Therapeutics
Russian Federation
Russian Federation
Cand. of Med. Sci., Research Officer of Central Scientific Research Laboratory, Doctoral Candidate of the Department of Hospital Surgery of Siberian State Medical University
A. N. Baykov
Central Scientific Research Laboratory
Russian Federation
Russian Federation
Doct. of Med. Sci., Professor, Head of Central Scientific Research Laboratory of State-Funded Educational Institution of Higher Professional Education Siberian State Medical University
G. Ts. Dambayev
Chair of Hospital Surgery Faculty of Therapeutics
Russian Federation
Russian Federation
Doct. of Med. Sci., Professor, Corresponding-Member of RAS, Head of the Chair of Hospital Surgery of State-Funded Educational Institution of Higher Professional Education Siberian State Medical University
P. S. Bushlanov
Chair of Surgical Diseases, Faculty of Pediatrics of Siberian State Medical University, Russian Ministry of Health
Russian Federation
Russian Federation
Resident of Chair of Surgical Diseases, Faculty of Pediatrics of State-Funded Educational Institution of Higher Professional Education Siberian State Medical University
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Review
For citations:
Semichev E.V., Baykov A.N., Dambayev G.Ts., Bushlanov P.S. Early Morphofunctional Changes in Rats' Liver after Left Lobe Resection using Cold Plasma Coagulator (Experimental Research). Annaly khirurgicheskoy gepatologii = Annals of HPB Surgery. 2015;20(1):76-83. (In Russ.) https://doi.org/10.16931/1995-5464.2015176-83
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