THE USE OF COMPOSITE MATERIAL LITAR TO CORRECT LIVER RESIDUAL CAVITIES

Aim. Experimental and histological study the possibility of using composite material “LitAr” for sealing and elimination of residual liver cavities in combination with oxytocin including in case of infection.

Material and Methods. All studies were performed on 69 white laboratory male rats of Wistar line with weight of 280–300 g. 6 series of experiments were conducted. All operations were performed under ether mask anesthesia in compliance with the rules of asepsis and antisepsis. The animals were sacrificed by overdose of ether in 3, 7, 14 and 30 days after the start of the experiment. The site of composite material “LitAr” implantation was dissected for further study using light microscopy, immunocytochemical (identification of pro- and antiapoptotic genes p53, Bcl-2, caspasa-3, proliferative protein Ki-67 expression) and electron microscopic analysis.

Results. Implantation of composite material “LitAr” into liver residual cavity stimulates reparative histogenesis that leads to partial filling of defect by connective tissue elements and organotypic structures (newly formed liver cells), but does not limit the development of purulent-necrotic processes in infected residual liver cavity. The use of oxytocin in filling of residual liver cavity with “LitAr” material provides adequate conditions for the implementation of histo chemically potencies of parenchymal and stromal structures. It results complete replacement of cavity by organotypic regenerator. The use of oxytocin in case of infected residual cavity limits the development of purulent-necrotic processes, limits the area of necrosis from viable tissues, optimizes reparative potency of liver tissues. So replacement of cavity by organotypic regenerator occurs. This morphological effect is particularly marked if oxytocin and antibiotics are used simultaneously.

Conclusion. Thus, the use of collagen composite “LitAr” combined with oxytocin and antibiotics provides the most positive influence on reparative histogenesis in histostructures of liver and bile ducts including in case of infection of cavity. It results active proliferation of undifferentiated tissue, regenerative hypertrophy of hepatocytes in the area adjacent to the cavity, increase of their mitotic activity and filling of residual cavity by connective tissue elements and organotypic structures.

1. Bulanov K.I. Modern aspects of surgical treatment of liver echinococcosis. Klinicheskaya khirurgiya. 2000; 3: 51–53. (In Russian)

2. Veronsky G.I., Ershov K.G., Popov А.I. Biliary fistulas as a complication of liver hydatid disease. Аnnaly khirurgicheskoy gepatologii. 2000; 5 (2): 302. (In Russian)

3. Vishnevsky V.A. Sovershenstvovanie metodov khirurgicheskogo lecheniya ochagovykh porazhenij pecheni [Improvement of surgical treatment of focal liver lesions]: author. dis. ... doct. med. sci. Moscow, 1990. 31 p. (In Russian)

4. Krasnov А.F., Litvinov S.D., Markov I.I. The use of “Litar” material in long bones defects substitution after osteomyelitis. Vestnik travmatologii i ortopedii im. N.I. Pirogova. 2004; 112 (4): 75–79. (In Russian)

5. Litvinov S.D., Bulanov S.I., Krasnov A.F. Collagen-Apatite material in jaw bone tissue defects substitution. Stomatologiya. 2001; 80 (3): 7–12. (In Russian)

6. Markov I.I., Litvinov S.D., Markov А.I. “LitAr” implant material induces angiogenesis. Morfologicheskie vedomosti. 2003; 1–2: 74–76. (In Russian)

7. Selyakin S.P., Antonova Yu.B. The use of collagen-hydroxiapatite material “Litar” in terms of reparative regeneration of lung tissue. Uspekhi sovremennogo estestvoznaniya. 2005; 12: 91–92. (In Russian)

8. Stadnikov А.А., Bukharin O.V. Morphological basis of hypothalamic neurosecretion influence on structural and functional homeostasis in pro- and eukaryotes. Morfologiya. 2013; 144 (5): 16–20. (In Russian)

9. Stadnikov А.А., Shevlyuk N.N., Kozlova А.N. O realizatsii apoptoznoj dominanty nejrosekretornykh kletok krupnokletochnykh yader gipotalamusa mlekopitayushhikh v usloviyakh infitsirovaniya organizma [On the implementation of apoptotic dominant of neurosecretory cells of hypothalamus large cell nuclei of mammals under organism's infection]. Proceedings of the 8th all-Russian scientific conference on pathology of the cell. Moscow: MDV. 2010: 238–240. (In Russian)

10. Pyshkin S.А., Rybakov V.A. Stimulation of regeneration in chronic hepatitis and liver cirrhosis management. Аnnaly khirurgicheskoy gepatologii. 2004; 9 (1): 60–68. (In Russian)