ATOMIC FORCE MICROSCOPY OF THE ERYTHROCYTE IN VARIOUS SEVERITY OF OBSTRUCTIVE JAUNDICE (EXPERIMENTAL STUDY)

Aim. To study morphofunctional peculiarities of erythrocyte depending on the severity of jaundice. Materials and Methods. Obstructive jaundice was modeled in 48 pigs. Morphological and functional characteristics of red blood cells were studied using atomic force microscope INTEGRA NT-MDT (Russia, Zelenograd). Results. The study proved that erythrocytes transformation is associated with severity of jaundice. In peripheral blood erythrocytes with clearly contoured membrane and homogeneous cytoplasm are observed along with those of domical shape and with spinous outgrowths on the membrane. Average roughness of red blood cells surface decreases from 40-44 to 26-30 nm2 , dispersion – from 112 to 84 nm2 , violations of membrane integrity increased. Erythrocyte’s volume increased up to 12.3 ± 2.6 mm3 , intracellular pressure – from 0 kPa in normal animals up to 2.2 kPa in animals with obstructive jaundice class C (by E.I. Galperin, 2012) that perhaps contributes to destruction of erythrocytes. Conclusion. Erythrocytes are extremely convenient model to study the effect of exogenous and endogenous factors due to their structural and physiological characteristics, as well as the availability to research them. So red blood cell may be used as an informative test object to assess the state of organism in various pathological processes including acute liver failure in obstructive jaundice. Moreover, features of pathogenesis, morphogenesis and optimal management may be determined.

obstructive jaundice, liver failure, atomic force microscopy, red blood cell’s membrane

1. Vinnik Yu.S., Pakhomova R.A., Kochetova L.V. Early results of treatment of patients with obstructive jaundice. Mezhdunarodnyj nauchno-prakticheskij zhurnal. Khirurgiya (Vostochnaya Evropa). 2012; 3 (03): 31–33. (In Russian)

2. Galperin E.I. Classification of obstructive jaundice severity. Annaly khirurgicheskoy gepatologii. 2012; 17 (2): 5–9. (In Russian)

3. Novitskiy V.V., Ryazantseva N.V., Stepovaya E.A., Bystritskiy L.D., Tkachenko S.B. Klinicheskij patomorfoz ehritrocita. Atlas [Clinical erythrocyte pathomorphosis. Atlas]. Tomsk: Publishing house of Tomsk University; Moscow: GEOTAR-med, 2003. 208 p. (In Russian)

4. Novitskiy V.V., Ryazantseva N.V., Stepovaya E.A. Fiziologiya i patofiziologiya ehritrocita [Physiology and pathophysiology of the erythrocyte]. Tomsk: Publishing house of Tomsk University, 2004. 200 p. (In Russian)

5. Skorkina M.Yu., Chernyavskikh S.D., Fedorova M.Z., Sladkova E.A., Zabinyakov N.A. Methods of evaluation of morphometric parameters of native blood cells by atomic force microscopy. Byulleten' ehksperimental'noj biologii i mediciny. 2010; 150 (2): 273–275. (In Russian)

6. Skorkina M.Yu., Chernyavskikh S.D., Fedorova M.Z., Sladkova E.A., Zabinyakov N.A. Comparative assessment of morphological and functional characteristics of native and fixed erythrocytes. Citologiya. 2011; 53 (1): 17–21. (In Russian)

7. Novoderzhkina Yu.K., Shishkanova Z.G., Kozinets G.I. Konfigu racija i poverhnost' kletok krovi v norme i patologii [Configuration and the surface of the red blood cells in norm and pathology]. Moscow: Triada-Farm, 2004. 152 p. (In Russian)

8. Nagornov Yu.S., Pakhomova R.A., Zhilyaev I.V., Voronova E.A. The morphology of the erythrocyte and intracellular pressure calculation according to atomic force microscopy. Rossijskij zhurnal biomekhaniki. 2015; 19 (4): 1–10. (In Russian)

9. Nagornov Yu.S., Pakhomova R.A. Atomic force microscopy of red blood cells membrane in obstructive jaundice. Biofisika. 2016; 61 (3): 483–491. (In Russian)